ADVANTAGE MALARIA CARD

Skip Navigation LinksHome > All Products > Rapid Test Kits > MALARIA Range

 

INTENDED USE

ADVANTAGE MALARIA CARD is a visual, rapid and sensitive immunoassay for the qualitative differential detection of P.falciparum (HRP-2) and P.vivax (pLDH) malaria antigen in human blood only.

SALIENT FEATURES

  • Rapid, visual antigen based test for detecting infection with P.falciparum (HRP-2) & P.vivax (pLDH) malaria parasite in whole blood.
  • Excellent Sensitivity & Specificity.
  • See through Device for easy result interpretation.
  • Longer shelf life of 24 months at 4-30° C.
  • Easy to interpret colour bands
  • Results within 20 minutes.
ADVANTAGE MALARIA CARD is an immunoassay based on the "Sandwich principle". Colloidal gold is conjugated to P.f. specific monoclonal anti-HRP-2 antibody and monoclonal anti-pan specific pLDH (plasmodium lactate dehydrogenase) antibody. The test uses monoclonal anti-Pf HRP-2 antibody (test line F) & monoclonal anti-P.v specific pLDH antibody (test line V) immobilized on a nitrocellulose strip. The test sample is added to the device. On addition of assay buffer, the red blood cells get lysed. If the sample contains P.falciparum or P.vivax or both, the colloidal gold conjugate complexes the HRP-2 / P.vivax specific pLDH in the lysed sample. This complex migrates through the nitrocellulose strip by capillary action. When the complex meets the line of the corresponding immobilized antibody, the complex is trapped forming a purplish pink band which confirms a reactive test result. Absence of a coloured band in the test region indicates a negative test result. To serve as a procedural control an additional line of anti-mouse antibody has been immobilized on the strip as control.
ORDERING INFORMATION
PRODUCT NAME PACK SIZE CATALOG NO.
ADVANTAGE MALARIA CARD 25 Tests IR211025

EVALUATIONS
  • PERFORMANCE CHARACTERISTICS (INTERNAL EVALUATION) OF ADVANTAGE MAL CARD
    The performance of ADVANTAGE MALARIA CARD test kit has been evaluated in house with positive & negative clinical samples tested by the microscopic examination of whole blood. The results obtained are as follows:
    Sample Total no of samples tested ADVANTAGE MALARIACARD Sensitivity Specificity
        Positive Negative % %
    Malaria Negative 450 1 449 - 99.77
    P.falciparum Positive 50 50 0 100 -
    P.vivax Positive 60 60 0 100 -

    PRECISION:Within-run and between-run precisions have been determined by testing 10 replicates of three specimens: a negative, a low positive and a strong positive. The C.V (%) of negative, low positive and strong positive values were within 10% of the time.


    PERFORMANCE EVALUATION

    All India Institute of Medical Sciences New Delhi.

  • AIIMS-New Delhi-As per evaluation at AIIMS with 200 fresh blood samples from random donors with known positive samples indicated 100 % sensitivity for both P.falciparum & P. vivax and 100 % specificity.

© COPYRIGHT 2015 ALL RIGHTS RESERVED. LEGAL DISCLAIMER.