ANTI-H LECTIN

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INTENDED USE

The Anti-H Lectin is a purified extract from seeds of Ulex europaeus. The reagent contains a phytohaemagglutinin which is virtually specific for the H antigen on human red blood cells. Anti-H Lectin is used to demonstrate the presence of H antigen on human red blood cells and in assessing the H secretor status of group 'O' individuals.

PRINCIPLE OF THE TEST

Major percentage of the population (80%) has soluble ABO blood group antigens present in their body fluids. These patients are known as ABH secretors while remaining 20% are non secretors. Secretors contain the following ABH antigens in their body fluids:


O  Secretors  :  Only H        :  A  Secretors     :  A & H
B  Secretors  :  B & H          :  AB  Secretors  :  A,B & H
Non  Secretors : None

Human red blood cells possessing H antigen will agglutinate in the presence of seed extract of Ulex europaeus containing phytoagglutinin specifically directed towards it. Saliva also contains water soluble 'H' antigen which gets neutralized by Anti-H Lectin. Agglutination of red cells/neutralization of Anti-H Lectin by saliva is a positive test result and indicates the presence of H substance on/in the red cells/saliva respectively. No agglutination of red cells/neutralization of Anti - H Lectin by saliva is a negative test result and indicates the absence of H substance on/in the red cells/saliva respectively.

STABILITY

Anti H Lectin is stable at 2-8°C for 24 months.

ORDERING INFORMATION
PRODUCT NAME PACK SIZE CATALOG NO.
Anti-H Lectin 5 ml BR080005

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